5JJD

crystal structure of the ceramide transfer protein PH and START domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.761, 60.914, 95.804
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.970 - 2.403
R-factor	0.2033
Rwork	0.201
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e3m 4hhv
RMSD bond length	0.003
RMSD bond angle	0.483
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	38.970
High resolution limit [Å]	2.400
Number of reflections	13860
<I/σ(I)>	19.9
Completeness [%]	97.7
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1 M MES bis-tris (pH 6.0), 6% (w/v) polyethylene glycol 10,000, and 10 micro molar calcium chloride