5JJ7

Fic-1 (aa134 - 508 E274G) from C. elegans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	H 3 2
Unit cell lengths	192.956, 192.956, 182.695
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	96.478 - 3.749
R-factor	0.2194
Rwork	0.216
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ed8
RMSD bond length	0.006
RMSD bond angle	1.162
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	96.478
High resolution limit [Å]	3.749
Number of reflections	13578
<I/σ(I)>	11.5
Completeness [%]	99.4
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	1.1M Ammonium Sulfate, 0.1M MES pH 6.5