5JJ3
Refined Structure of the Mature Virion Conformation of P22 Portal Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-14 |
| Detector | RAYONIX MX325HE |
| Wavelength(s) | 0.90 |
| Spacegroup name | I 4 |
| Unit cell lengths | 409.043, 409.043, 260.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.993 - 7.000 |
| R-factor | 0.2401 |
| Rwork | 0.239 |
| R-free | 0.25970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lj5 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.014 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX ((1.10.1_2155: ???)) |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 7.000 |
| Number of reflections | 33729 |
| <I/σ(I)> | 19.4 |
| Completeness [%] | 98.8 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 30% tert-butanol, 70 mM sodium chloride, 2.5% PEG400 in 0.1 M sodium acetate |
