5JJ1

Structure of the Immature Procapsid Conformation of P22 Portal Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-21
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97
Spacegroup name	P 42
Unit cell lengths	316.808, 316.808, 138.618
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.998 - 3.300
R-factor	0.2952
Rwork	0.295
R-free	0.31470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.995
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.300
Number of reflections	206085
<I/σ(I)>	10.7
Completeness [%]	100.0
Redundancy	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	5% PEG 8,000, 10 mM Cesium Chloride