5JIW
Crystal structure of Thermus aquaticus amylomaltase (GH77) in complex with a 34-meric cycloamylose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91841 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 157.600, 157.600, 112.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.150 - 1.730 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cwy |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.807 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.200 |
| High resolution limit [Å] | 1.730 |
| Number of reflections | 74016 |
| <I/σ(I)> | 16.9 |
| Completeness [%] | 99.3 |
| Redundancy | 18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.0 M NaKphosphate pH 7 |
