5JIU

The crystal structure of RanBPM/9 IUS-SPRY domain in complex with DDX-4 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	I 4
Unit cell lengths	123.318, 123.318, 91.986
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.058 - 2.054
R-factor	0.1678
Rwork	0.166
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ji7
RMSD bond length	0.008
RMSD bond angle	1.068
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.050	2.050
Number of reflections	42894
<I/σ(I)>	22.2658
Completeness [%]	97.3
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	20~25% (w/v) PEG 3350, 0.1 M Bis-Tris, pH 6.5, 0.2 M NaCl