5JI9

The Crystal Structure Of IUS-SPRY Domain From RanBPM/9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-10
Detector	ADSC QUANTUM 210
Wavelength(s)	1.10000
Spacegroup name	P 65
Unit cell lengths	69.478, 69.478, 107.688
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.120 - 2.500
R-factor	0.173
Rwork	0.167
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ji7
RMSD bond length	0.008
RMSD bond angle	1.168
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge		0.284
Number of reflections	10287
<I/σ(I)>	22.0625
Completeness [%]	99.3	95.8
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	0.1M NA-CACODYLATE, PH 6.5, 6% PEG 4000, 0.2M KCL, 0.02M MGCL2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 295K