5JHZ
Crystal Structure of Fungal MagKatG2 at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9729 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.200, 109.380, 133.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.15713 |
| Rwork | 0.156 |
| R-free | 0.18643 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ut2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.437 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.075 | 0.075 |
| Number of reflections | 161673 | |
| <I/σ(I)> | 14.6 | 2.7 |
| Completeness [%] | 97.5 | 96.6 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 15% PEG4000, 0.1 M sodium acetate, pH 4.6 (+ pH 7 phosphate soaking) |
