5JHV
Apo form of influenza strain H1N1 polymerase acidic subunit N-terminal region crystallized with polyethylene glycol 8000
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.243, 87.404, 66.497 |
| Unit cell angles | 90.00, 94.31, 90.00 |
Refinement procedure
| Resolution | 36.490 - 2.749 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.23440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zqq |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.676 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.749 | 2.749 |
| Rmerge | 0.159 | 0.635 |
| Number of reflections | 9578 | |
| <I/σ(I)> | 8.91 | 2.255 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M Tris-HCl pH 7.5, 28% PEG 8000, 0.2 M Calcium acetate, 10% Jeffamine M-600 |
