5JHO
Crystal structure of the regulatory domain of the sodium driven chloride bicarbonate exchanger.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 98.040, 98.040, 212.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.988 - 2.801 |
| R-factor | 0.2022 |
| Rwork | 0.200 |
| R-free | 0.23780 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.394 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.320 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.053 | 1.000 |
| Number of reflections | 26378 | |
| <I/σ(I)> | 30.5 | 2.5 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 10.9 | 10.9 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 277 | 40 mg/ml protein, 100 mM Formic Acid , pH 4.4, 9 % PEG 8000 |
