5JHN
Structure of G9a SET-domain with Histone H3K9Ala mutant peptide and bound S-adenosylmethionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 175 |
| Detector technology | CCD |
| Collection date | 2012-01-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.010 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.782, 78.270, 73.121 |
| Unit cell angles | 90.00, 90.34, 90.00 |
Refinement procedure
| Resolution | 34.500 - 1.670 |
| R-factor | 0.21034 |
| Rwork | 0.209 |
| R-free | 0.23740 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.884 |
| Data reduction software | HKL-2000 (2000) |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.500 |
| High resolution limit [Å] | 1.670 |
| Number of reflections | 70175 |
| <I/σ(I)> | 16.67 |
| Completeness [%] | 99.2 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 0.2 M Tri-ammonium citrate pH 7 and 20% w/v polyethylene glycol 3350 |
