5JHM
Crystal structure of Zika virus Envelope protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.448, 59.063, 103.669 |
| Unit cell angles | 90.00, 103.72, 90.00 |
Refinement procedure
| Resolution | 33.983 - 2.000 |
| R-factor | 0.2096 |
| Rwork | 0.208 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oke |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.151 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 57290 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 93.3 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 magnesium acetate tetrahydrate, 0.1 sodium cacodylate, pH 6.5, 20 % w/v PEG 8000 |
