5JHK
X-ray structure of neuropilin-1 b1 domain complexed with Arg-6 ligand.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.376, 90.781, 40.965 |
| Unit cell angles | 90.00, 98.27, 90.00 |
Refinement procedure
| Resolution | 45.390 - 1.800 |
| R-factor | 0.16957 |
| Rwork | 0.167 |
| R-free | 0.21509 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kex |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.731 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.540 | 1.670 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.064 | 1.393 |
| Number of reflections | 36381 | |
| <I/σ(I)> | 18.9 | 1.2 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 6.7 | 6.8 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 293 | 30%PEG3350+0,2M NH4Cl |
