5JHF
Crystal structure of Atg13(17BR)-Atg13(17LR)-Atg17-Atg29-Atg31 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-11-21 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 147.956, 64.043, 184.387 |
| Unit cell angles | 90.00, 109.91, 90.00 |
Refinement procedure
| Resolution | 49.220 - 3.210 |
| R-factor | 0.22596 |
| Rwork | 0.224 |
| R-free | 0.26510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p1w |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.726 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 53805 | |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 5% polyethylene glycol monomethyl ether 5000, 100 mM Tris-HCl |
