5JH9
Crystal structure of prApe1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2006-10-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 140.428, 140.428, 348.922 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.790 - 2.100 |
| R-factor | 0.18332 |
| Rwork | 0.181 |
| R-free | 0.20737 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jgf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.208 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.045 | 0.190 |
| Number of reflections | 123984 | |
| <I/σ(I)> | 20.3 | 5.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 50% (v/v) PEG 200, 200 mM Magnesium chloride, 100 mM sodium cacodylate |
