5JH0
Crystal structure of the mitochondrial DNA packaging protein Abf2p in complex with DNA at 2.18 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-09-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979500 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.490, 113.410, 67.710 |
| Unit cell angles | 90.00, 103.80, 90.00 |
Refinement procedure
| Resolution | 43.483 - 2.180 |
| R-factor | 0.2106 |
| Rwork | 0.209 |
| R-free | 0.23640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PARTIAL MODEL OBTAINED WITH SINGLE ANOMALOUS DIFFRACTION |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.948 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.10.1_2155) |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.483 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.066 | |
| Number of reflections | 35516 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 96.1 | 68.3 |
| Redundancy | 3.7 | 1.9 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 21% PEG 4000, 0.1 M Trisodium Citrate, 0.2 M Ammonium Acetate |
