5JH0

Crystal structure of the mitochondrial DNA packaging protein Abf2p in complex with DNA at 2.18 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	80
Detector technology	CCD
Collection date	2013-09-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979500
Spacegroup name	C 1 2 1
Unit cell lengths	88.490, 113.410, 67.710
Unit cell angles	90.00, 103.80, 90.00

Refinement procedure

Resolution	43.483 - 2.180
R-factor	0.2106
Rwork	0.209
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PARTIAL MODEL OBTAINED WITH SINGLE ANOMALOUS DIFFRACTION
RMSD bond length	0.008
RMSD bond angle	0.948
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.10.1_2155)
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.483	2.180
High resolution limit [Å]	2.120	2.120
Rmerge	0.066
Number of reflections	35516
<I/σ(I)>	11.3
Completeness [%]	96.1	68.3
Redundancy	3.7	1.9
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	21% PEG 4000, 0.1 M Trisodium Citrate, 0.2 M Ammonium Acetate