5JGY

Crystal structure of maize AKR4C13 in P21 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-09
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9334
Spacegroup name	P 1 21 1
Unit cell lengths	53.750, 115.301, 56.466
Unit cell angles	90.00, 104.28, 90.00

Refinement procedure

Resolution	57.650 - 1.450
R-factor	0.1279
Rwork	0.126
R-free	0.16270
RMSD bond length	0.008
RMSD bond angle	1.597
Data scaling software	XSCALE
Phasing software	BALBES
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.650		1.540
High resolution limit [Å]	1.450	4.320	1.450
Rmerge	0.048	0.016	0.644
Number of reflections	230943
<I/σ(I)>	11.37	43.36	1.4
Completeness [%]	98.6	95.1	98.6
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	15% (w/v) PEG 1500 and 0.1 M SPG buffer pH 4.5 added by 0.01 M of beta-NAD