5JGU

Spin-Labeled T4 Lysozyme Construct R119V1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 32 2 1
Unit cell lengths	59.919, 59.919, 96.154
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.666 - 1.468
R-factor	0.1527
Rwork	0.151
R-free	0.17460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c6t
RMSD bond length	0.008
RMSD bond angle	0.781
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.670
High resolution limit [Å]	1.468	1.468
Number of reflections	33872
<I/σ(I)>	25.02
Completeness [%]	97.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	297	sodium/potassium phosphate, sodium chloride, hexane-1,6-diol, 2-propanol