5JGQ
X-ray structure of neuropilin-1 b1 domain complexed with Arg-7 ligand.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.789, 89.568, 41.385 |
| Unit cell angles | 90.00, 98.57, 90.00 |
Refinement procedure
| Resolution | 44.780 - 1.600 |
| R-factor | 0.16219 |
| Rwork | 0.161 |
| R-free | 0.19304 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kex |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.864 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.920 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.047 | 0.442 |
| Number of reflections | 37578 | |
| <I/σ(I)> | 10.5 | 2 |
| Completeness [%] | 97.0 | 75.3 |
| Redundancy | 3.1 | 1.9 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 289 | 18%PEG3350+0.2M NH4Cl |
