5JGH
Crystal structure of the mitochondrial DNA packaging protein Abf2p in complex with DNA at 2.6 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-04-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976250 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.989, 131.991, 71.994 |
| Unit cell angles | 90.00, 103.21, 90.00 |
Refinement procedure
| Resolution | 48.048 - 2.600 |
| R-factor | 0.2283 |
| Rwork | 0.227 |
| R-free | 0.26010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PARTIAL MODEL FROM SAD PHASING |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.996 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX ((1.10.1_2155) |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.050 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.112 | |
| Number of reflections | 38389 | |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 95.0 | 95.3 |
| Redundancy | 2.3 | 2.3 |
| CC(1/2) | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 20% PEG 4000, 0.2 M Ammonium Acetate, 0.1 M Tri-sodium Citrate pH 4.5 |
