5JGA
Crystal structure of human TAK1/TAB1 fusion protein in complex with ligand 11c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-03-17 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 58.009, 133.796, 141.374 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 97.180 - 2.000 |
| R-factor | 0.1965 |
| Rwork | 0.195 |
| R-free | 0.22130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eva |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.353 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 97.180 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.067 | 0.026 | 0.542 |
| Number of reflections | 36658 | ||
| <I/σ(I)> | 11.6 | ||
| Completeness [%] | 97.8 | 99.6 | 87.9 |
| Redundancy | 7.1 | 7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 294 | 1.7M sodium potassium phosphate, 20%(v/v) Glycerol as cryoprotectant |
