5JFY
Crystal structure of a plant cytidine deaminase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-20 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97949 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 99.808, 99.808, 143.061 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.635 - 2.101 |
R-factor | 0.1822 |
Rwork | 0.181 |
R-free | 0.21360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3j |
RMSD bond length | 0.003 |
RMSD bond angle | 0.500 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.670 | 47.670 | 2.160 |
High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
Rmerge | 0.080 | 0.020 | 0.607 |
Total number of observations | 101431 | 1338 | 6639 |
Number of reflections | 41687 | ||
<I/σ(I)> | 9.7 | 38.9 | 1.4 |
Completeness [%] | 97.9 | 84.9 | 95 |
Redundancy | 2.4 | 2.5 | 2 |
CC(1/2) | 0.997 | 0.999 | 0.622 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293.15 | 0.0085 M CoCl2, 0.085 sodium acetate pH 4.6, 0.85 M 1,6-hexanediol, 15% (v/v) glycerol |