5JFQ

Geranylgeranyl Pyrophosphate Synthetase from archaeon Geoglobus acetivorans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-15
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	85.860, 85.860, 188.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.112 - 2.510
R-factor	0.2077
Rwork	0.205
R-free	0.25880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wy0
RMSD bond length	0.009
RMSD bond angle	1.009
Data reduction software	MOSFLM
Phasing software	BALBES
Refinement software	PHENIX ((dev_2405: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.080	2.620
High resolution limit [Å]	2.510	2.510
Rmerge	0.309
Number of reflections	24891
<I/σ(I)>	6.7
Completeness [%]	99.8	99.7
Redundancy	19.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	310	0.1M Tris pH 8.0, 0.1M sodium malonate pH 8.0, 33% PEG 1000 and 10% ethylene glycol mixed with protein in ratio 1:1 over 500 ml of precipitant solution covered with oils