5JEE
Apo-structure of humanised RadA-mutant humRadA26F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96860 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.581, 50.597, 74.269 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.130 - 1.490 |
| R-factor | 0.1951 |
| Rwork | 0.194 |
| R-free | 0.21830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j4l |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.269 | 1.492 |
| High resolution limit [Å] | 1.487 | 1.487 |
| Rmerge | 0.063 | 0.844 |
| Number of reflections | 31823 | |
| <I/σ(I)> | 17.5 | 2.2 |
| Completeness [%] | 99.3 | 87.6 |
| Redundancy | 6.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1M NaAcetate pH=4.6, 15% PEG20K |
