5JED
Apo-structure of humanised RadA-mutant humRadA28
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96862 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.771, 72.100, 41.927 |
| Unit cell angles | 90.00, 115.89, 90.00 |
Refinement procedure
| Resolution | 37.720 - 1.332 |
| R-factor | 0.13669 |
| Rwork | 0.135 |
| R-free | 0.17122 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j4l |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.579 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.720 | 1.337 |
| High resolution limit [Å] | 1.332 | 1.332 |
| Rmerge | 0.093 | 1.086 |
| Number of reflections | 45801 | |
| <I/σ(I)> | 7.6 | 2.1 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 4.7 | 3.2 |
| CC(1/2) | 0.992 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.1M NaCacodylate pH=6.5, 5% PEG8000, 40% MPD |
