5JE6
Crystal structure of Burkholderia glumae ToxA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.977 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.992, 56.424, 60.831 |
| Unit cell angles | 90.00, 91.32, 90.00 |
Refinement procedure
| Resolution | 19.400 - 1.571 |
| R-factor | 0.1491 |
| Rwork | 0.147 |
| R-free | 0.17890 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.414 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((1.10-2155-000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.400 | 1.630 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.027 | 0.215 |
| Number of reflections | 69075 | |
| <I/σ(I)> | 21.5 | 4.3 |
| Completeness [%] | 95.6 | 89.3 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 19% polyethylene glycol 3000 and 100 mM sodium citrate, pH 5.5 |
