5JBV
Lys27-linked triubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-28 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 51.758, 51.758, 138.252 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.565 - 2.104 |
| R-factor | 0.2634 |
| Rwork | 0.261 |
| R-free | 0.30610 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.634 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.565 |
| High resolution limit [Å] | 2.104 |
| Number of reflections | 10189 |
| <I/σ(I)> | 7.01 |
| Completeness [%] | 85.8 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 291 | 20% (w/v) PEG 3350, 4mM CdCl2, pH 5.9, 200mM Mg(NO3)2 |
