5JBR

Crystal structure of uncharacterized protein Bcav_2135 from Beutenbergia cavernae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-17
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9793149
Spacegroup name	P 1
Unit cell lengths	45.096, 46.461, 47.820
Unit cell angles	104.04, 102.34, 117.67

Refinement procedure

Resolution	35.433 - 1.650
R-factor	0.1554
Rwork	0.153
R-free	0.19940
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.710
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (dev_2328)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.680
High resolution limit [Å]	1.650	4.480	1.650
Rmerge	0.041	0.028	0.517
Number of reflections	36092
<I/σ(I)>	11.8
Completeness [%]	97.2	97.3	95.3
Redundancy	3.9	3.8	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	289	Lithium sulfate, sodium acetate