5JBR
Crystal structure of uncharacterized protein Bcav_2135 from Beutenbergia cavernae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-17 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9793149 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.096, 46.461, 47.820 |
| Unit cell angles | 104.04, 102.34, 117.67 |
Refinement procedure
| Resolution | 35.433 - 1.650 |
| R-factor | 0.1554 |
| Rwork | 0.153 |
| R-free | 0.19940 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.710 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (dev_2328) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 4.480 | 1.650 |
| Rmerge | 0.041 | 0.028 | 0.517 |
| Number of reflections | 36092 | ||
| <I/σ(I)> | 11.8 | ||
| Completeness [%] | 97.2 | 97.3 | 95.3 |
| Redundancy | 3.9 | 3.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | Lithium sulfate, sodium acetate |
