5JBO

Trichoderma harzianum GH1 beta-glucosidase ThBgl2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 QUEST
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-01
Detector	APEX II CCD
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	57.519, 78.142, 100.272
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.640 - 1.970
R-factor	0.1698
Rwork	0.168
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ahy
RMSD bond length	0.003
RMSD bond angle	0.601
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1-2155_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	61.640
High resolution limit [Å]	1.970
Number of reflections	32663
<I/σ(I)>	8.7
Completeness [%]	100.0
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 MES pH 6.5, 25% PEG 8000