5JAK

Crystal structure of the flagellar assembly factor FliW

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-06
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.87260
Spacegroup name	P 2 21 21
Unit cell lengths	31.237, 77.990, 87.048
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.524 - 1.801
R-factor	0.2279
Rwork	0.227
R-free	0.24480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dmd
RMSD bond length	0.012
RMSD bond angle	1.473
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	43.524
High resolution limit [Å]	1.801
Rmerge	0.052
Number of reflections	20404
<I/σ(I)>	19.21
Completeness [%]	99.7
Redundancy	7.4
CC(1/2)	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M sodium acetate, 0.1 M sodium cacolydate pH 6.5, 30 % (w/v) PEG 8000