5J9T

Crystal structure of the NuA4 core complex

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	173
Detector technology	CCD
Collection date	2014-12-27
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	86.685, 137.582, 168.076
Unit cell angles	90.00, 97.63, 90.00

Refinement procedure

Resolution	29.971 - 2.700
R-factor	0.1877
Rwork	0.187
R-free	0.21930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.704
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.700
Number of reflections	106451
<I/σ(I)>	12.9
Completeness [%]	99.8
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	100mM HEPES (pH 7.5), 6% 1,6-Hexanediol, 7% PEG 8000, 5% ethylene glycol, 10mM DTT.