5J9I
Crystal structure of the HigA2 antitoxin C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.980110 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.360, 61.160, 73.850 |
| Unit cell angles | 90.00, 106.69, 90.00 |
Refinement procedure
| Resolution | 38.507 - 1.797 |
| R-factor | 0.1961 |
| Rwork | 0.194 |
| R-free | 0.23570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.362 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.854 | 45.854 | 1.910 |
| High resolution limit [Å] | 1.797 | 5.360 | 1.800 |
| Rmerge | 0.053 | 0.036 | 0.409 |
| Number of reflections | 44925 | ||
| <I/σ(I)> | 12.88 | 27.25 | 2.27 |
| Completeness [%] | 97.5 | 97.9 | 91.4 |
| Redundancy | 3.99 | ||
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Tris HCl pH 8.5, 200 mM calcium chloride, 20% PEG 4000 |
