5J9G

Structure of Lactobacillus acidophilus glyceraldehyde-3-phosphate dehydrogenase at 2.21 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-03-25
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 41 21 2
Unit cell lengths	114.990, 114.990, 113.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.330 - 2.210
R-factor	0.20427
Rwork	0.203
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qx6
RMSD bond length	0.012
RMSD bond angle	1.525
Data reduction software	iMOSFLM (1.0.7)
Data scaling software	Aimless (0.2.1)
Phasing software	PHASER (2.5.5)
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.330
High resolution limit [Å]	2.210
Rmerge	0.093
Number of reflections	38437
<I/σ(I)>	16.5
Completeness [%]	99.9	97.3
Redundancy	17.2
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.4	295	10% w/v PEG 1000, 10% w/v PEG 8000