5J93
Five minutes iron loaded Rana Catesbeiana H' ferritin variant E57A/E136A/D140A
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000, 1.722, 1.759 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 183.782, 183.782, 183.782 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.950 - 1.100 |
| R-factor | 0.13026 |
| Rwork | 0.130 |
| R-free | 0.14264 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j8s |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.306 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.950 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.058 | 0.329 |
| Number of reflections | 104724 | |
| <I/σ(I)> | 19.1 | 3.5 |
| Completeness [%] | 97.8 | 84.9 |
| Redundancy | 9.9 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281 | 1.6-2.0 M MgCl2 and 0.1 M bicine pH 9.0 |
