5J8S
Iron-free state of Rana Catesbeiana H' ferritin variant E57A/E136A/D140A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-25 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0039 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 185.010, 185.010, 185.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.770 - 1.500 |
| R-factor | 0.16077 |
| Rwork | 0.160 |
| R-free | 0.17884 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lqh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.263 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.250 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.086 | 0.398 |
| Number of reflections | 43845 | |
| <I/σ(I)> | 14.5 | 4.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.7 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281 | 1.6-2.0 M MgCl2 and 0.1 M bicine pH 9.0 |
