5J8R
Crystal Structure of the Catalytic Domain of Human Protein Tyrosine Phosphatase non-receptor Type 12 - K61R mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-23 |
Detector | SYNTEX |
Wavelength(s) | 0.98 |
Spacegroup name | H 3 |
Unit cell lengths | 129.938, 129.938, 278.757 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.791 - 2.043 |
R-factor | 0.2406 |
Rwork | 0.239 |
R-free | 0.27520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qcj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.130 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.800 | 2.116 |
High resolution limit [Å] | 2.000 | 2.043 |
Number of reflections | 100516 | |
<I/σ(I)> | 55.4 | |
Completeness [%] | 99.5 | |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | 3.5M Sodium formate |