5J8N

Exonuclease III homologue Mm3148 from Methanosarcina mazei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-16
Detector	MAR CCD 165 mm
Wavelength(s)	0.81500
Spacegroup name	P 21 21 21
Unit cell lengths	46.726, 64.564, 84.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.831 - 1.440
R-factor	0.148
Rwork	0.147
R-free	0.17130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fzi
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	15.000	15.000	1.490
High resolution limit [Å]	1.440	3.090	1.440
Rmerge	0.052	0.041	0.274
Number of reflections	47242
<I/σ(I)>	13.3
Completeness [%]	99.9	99.5	100
Redundancy	6.3	5.9	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	20 % (w/v) PEG 8000, 20 % (w/v) PEG 400, 100 mM Tris/HCl pH 8.5, 100 mM MgCl2 mixed 1:1 with 10 mg/ml protein dissolved in 300 mM NaCl, 10 mM KHEPES pH 7.6, 2 mM DTT and addition of a seed stock solution (1/10 volume)