5J8B

Crystal structure of Elongation Factor 4 (EF-4/LepA) in complex with GDPCP bound to the Thermus thermophilus 70S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	213.124, 271.723, 436.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.521 - 2.600
R-factor	0.1953
Rwork	0.192
R-free	0.26090
RMSD bond length	0.007
RMSD bond angle	1.254
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1-2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.668	49.668	2.750
High resolution limit [Å]	2.590	7.690	2.590
Rmerge	0.257	0.047	1.517
Rmeas	0.290	0.054	1.730
Total number of observations	3199533
Number of reflections	766326	30209	118578
<I/σ(I)>	8.23	28.48	1.13
Completeness [%]	98.7	97.8	95.1
CC(1/2)	0.987	0.998	0.448

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME