5J83

Crystal structure of L-arabinonate dehydratase in apo-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.87260
Spacegroup name	P 64
Unit cell lengths	161.130, 161.130, 114.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.906 - 3.001
R-factor	0.2109
Rwork	0.208
R-free	0.25860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.541
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11_2567: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.906	3.100
High resolution limit [Å]	3.000	3.000
Number of reflections	33781
<I/σ(I)>	15.9	2
Completeness [%]	99.3	96.6
Redundancy	4.3	4.3
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	52% Tacsimate pH 7.0