5J7U
Faustovirus major capsid protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03318 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.380, 390.830, 130.140 |
| Unit cell angles | 90.00, 91.66, 90.00 |
Refinement procedure
| Resolution | 72.919 - 2.440 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j7o |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.606 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.170 | 2.500 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.230 | |
| Number of reflections | 317565 | |
| <I/σ(I)> | 8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.6 M ammonium sulfate, 10% dioxane |
