5J7R

2.5 Angstrom Crystal Structure of Putative Lipoprotein from Clostridium perfringens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-21
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 32 2 1
Unit cell lengths	118.212, 118.212, 71.836
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.550 - 2.500
R-factor	0.22126
Rwork	0.219
R-free	0.26771
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.601
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.550	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.064	0.618
Number of reflections	20303
<I/σ(I)>	49.7	4.3
Completeness [%]	99.9	100
Redundancy	9.2	9.5
CC(1/2)	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	292	Protein: 16.8 mg/ml, 0.1M Tris HCl (pH 8.3); Screen: JCSG+ (H11), 0.2M Magnesium chloride, 0.1M Bis-Tris pH 5.5, 25% (w/v) PEG 3350.