5J7K
Loop grafting onto a highly stable FN3 scaffold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.220, 71.230, 126.141 |
| Unit cell angles | 90.00, 90.43, 90.00 |
Refinement procedure
| Resolution | 50.219 - 2.460 |
| R-factor | 0.2611 |
| Rwork | 0.259 |
| R-free | 0.28540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u3h |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.058 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.220 | 2.560 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.083 | 1.170 |
| Number of reflections | 32118 | |
| <I/σ(I)> | 11.6 | 1.7 |
| Completeness [%] | 98.8 | 98.8 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 5% glycerol, 10% 2-propanol, 0.2 M zinc acetate, 0.1 M sodium cacodylate, pH 6.0 |
