5J76
Structure of Lectin from Colocasia esculenta(L.) Schott
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-20 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.471, 75.471, 121.177 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.760 - 2.100 |
| R-factor | 0.1665 |
| Rwork | 0.165 |
| R-free | 0.20440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mez |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.050 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.300 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 23782 | |
| <I/σ(I)> | 20.8 | |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 5.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4 | 293 | 2.7M Na Malonate |
