5J74
Fluorogen activating protein AM2.2 in complex with TO1-2p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-22 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9794 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.448, 114.830, 130.995 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.350 - 2.700 |
| R-factor | 0.19951 |
| Rwork | 0.197 |
| R-free | 0.25039 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b0s |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.493 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.350 | 2.760 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.138 | 0.578 |
| Number of reflections | 16728 | |
| <I/σ(I)> | 14.3 | 3.3 |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 6.1 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 32% PEG monomethyl ether 5000 0.2M potassium dihydrogen phosphate |
