5J70
The Chd1 DNA-binding domain in complex with 17mer DNA duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.076, 57.099, 99.493 |
| Unit cell angles | 90.00, 103.90, 90.00 |
Refinement procedure
| Resolution | 46.985 - 2.956 |
| R-factor | 0.2318 |
| Rwork | 0.231 |
| R-free | 0.25180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ted |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.662 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.553 | 3.140 |
| High resolution limit [Å] | 2.956 | 2.956 |
| Rmerge | 0.076 | 1.139 |
| Number of reflections | 19925 | |
| <I/σ(I)> | 20.3 | 1.89 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 7.44 | 7.48 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 277 | 14% PEG 4000, 0.2M ammonium acetate, 0.01M CaCl2, 0.05 M Na cacodylate, pH 5.5 |
