5J67
Structure of Astrotactin-2, a conserved vertebrate-specific and perforin-like membrane protein involved in neuronal development
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 |
| Unit cell lengths | 101.240, 108.190, 111.360 |
| Unit cell angles | 87.18, 84.50, 65.67 |
Refinement procedure
| Resolution | 98.580 - 3.160 |
| R-factor | 0.2347 |
| Rwork | 0.233 |
| R-free | 0.25855 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.357 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.580 | |
| High resolution limit [Å] | 3.160 | 3.160 |
| Rmerge | 0.093 | 0.890 |
| Number of reflections | 73362 | |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 99.9 | |
| Redundancy | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 12% PEG 20000, 150 mM KSCN, 0.1 M Bis-tris propane pH7.5 - 8.5 |
