5J5U
Fjoh_4561 chitin-binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 82.706, 82.885, 93.905 |
| Unit cell angles | 66.44, 78.33, 67.56 |
Refinement procedure
| Resolution | 44.490 - 2.300 |
| R-factor | 0.215 |
| Rwork | 0.214 |
| R-free | 0.26260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j90 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.135 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.490 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.123 | 0.306 |
| Number of reflections | 181362 | |
| <I/σ(I)> | 7.88 | 4.14 |
| Completeness [%] | 97.3 | 96.68 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PegRx screen #2, condition 48: 3% Dextran sulfate sodium salt, 0.1 M Bicine pH 8.5, 15% Poly(ethylene) glycol 20,000 |
