5J5Q
AMP-PNP-stabilized ATPase domain of topoisomerase IV from Streptococcus pneumoniae, complex type II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-28 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.950, 72.740, 222.960 |
| Unit cell angles | 90.00, 91.89, 90.00 |
Refinement procedure
| Resolution | 60.759 - 2.830 |
| R-factor | 0.1876 |
| Rwork | 0.184 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | derived from 1EI1 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.330 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.759 | 2.920 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Rmerge | 0.047 | 0.466 |
| Number of reflections | 48001 | |
| <I/σ(I)> | 2.5 | 1.5 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 3.31 | 3.3 |
| CC(1/2) | 0.975 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 5 mM MgCl2, 50 mM HEPES, 25% PEG MME 550 |
