5J5O
Translation initiation factor 4E in complex with m7GppppG mRNA 5' cap analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.990, 38.030, 146.640 |
| Unit cell angles | 88.55, 84.70, 76.67 |
Refinement procedure
| Resolution | 36.820 - 1.867 |
| R-factor | 0.2223 |
| Rwork | 0.221 |
| R-free | 0.26340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l8b |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.031 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.820 | 1.920 |
| High resolution limit [Å] | 1.867 | 1.867 |
| Rmerge | 0.071 | 0.555 |
| Number of reflections | 60259 | |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 91.2 | |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 13% PEG5000MME, 0.1M Tris |
