5J5N
Crystal structure of the R39W mutant of Populus trichocarpa glutathione transferase PtGSTU30 in complex with glutathione
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.355, 59.215, 79.973 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.603 - 2.629 |
R-factor | 0.1921 |
Rwork | 0.188 |
R-free | 0.26890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5j4u |
RMSD bond length | 0.009 |
RMSD bond angle | 1.281 |
Data reduction software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.650 |
Number of reflections | 14017 | |
<I/σ(I)> | 22.105 | 2 |
Completeness [%] | 99.2 | |
Redundancy | 6 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 291.15 | 30% (w/v) PEG 4000, 0.2M Ammonium acetate, 0.1M Sodium cacodylate trihydrate, pH 6.6 |